A Bioactive and Bioresorbable Porous Cubic Composite Scaffold Loaded With Bone Marrow Aspirate A Potential Alternative to Autogenous Bone Grafting

作者：Tanaka Kojiro^*; Takemoto Mitsuru; Fujibayashi Shunsuke; Neo Masashi; Shikinami Yasuo; Nakamura Takashi

来源：Spine, 2011, 36(6): 441-447.

DOI：10.1097/BRS.0b013e3181d39067

• posterolateral spinal fusion
• porous composite scaffold
• particulate hydroxyapatite/poly-DL-lactide
• bone marrow aspirate
• rabbit model

Study Design. Experimental animal study.
Objective. To investigate the osteogenic properties of a particulate uncalcined, unsintered hydroxyapatite/polydllactide (u-HA/PdlLA) composite scaffold loaded with bone marrow aspirate (BMA).
Summary of Background Data. Because of the high morbidity associated with bone graft harvesting, current research in spine surgery has largely focused on bone graft alternatives involving a combination of scaffolds and osteogenic substances. BMA is obtained by a simple and relatively noninvasive method and can easily be clinically applied as an osteogenic material. However, few studies have reported successful posterolateral spinal fusion (PLF) with BMA-loaded synthetic materials.
Methods. Porous u-HA/PdlLA composites loaded with BMA were used as bone graft substitutes. In experiment 1, porous u-HA/PdlLA cylinders containing or lacking BMA were implanted in rabbit muscles. They were retrieved 4, 8, and 12 weeks after implantation, and ectopic bone formation was histologically evaluated. In experiment 2, 48 rabbits underwent PLF with 1 of 4 bone grafts: autogenous bone (group 1); single-strip u-HA/PdlLA alone (group 2); morselized u-HA/PdlLA + BMA (group 3); or single-strip u-HA/PdlLA + BMA (group 4). After 12 weeks, fusion was assessed by manual palpation, microcomputed tomography, mechanical tests, and histologic examination.
Results. In experiment 1, ectopic bone formation was observed in BMA-loaded u-HA/PdlLA, and the new bone area increased until 12 weeks after implantation. In experiment 2, the fusion rates in groups 1, 2, 3, and 4 were 58.3%, 16.7%, 66.7%, and 91.7%, respectively, as determined by manual palpation, and 66.7%, 16.7%, 75.0%, and 91.7%, respectively, as determined by microcomputed tomography. The mechanical strength was significantly greater in group 4 than in the other groups (P < 0.05).
Conclusion. Conclusion. Our results indicate that BMA-loaded porous mu-HA/PdlLA is an effective alternative to autogenous bone grafts. The structure and composition of porous u-HA/PdlLA render it an effective scaffold for BMA.

• 出版日期2011-3-15