Differentiation of bone marrow stromal cells in poly(lactide-co-glycolide)/chitosan scaffolds

作者：Kuo Yung Chih^*; Yeh Chun Fu; Yang Jen Tsung

来源：Biomaterials, 2009, 30(34): 6604-6613.

DOI：10.1016/j.biomaterials.2009.08.028

• Bone marrow stromal cell
• Differentiation
• Poly(lactide-co-glycolide)
• Chitosan
• Neuron
• Osteoblast

This study investigates the physicochemical properties of poly(lactide-co-glycolide) (PLGA)/chitosan scaffolds and the neuron growth factor (NGF)-guided differentiation of bone marrow stromal cells (BMSCs) in the scaffolds. The scaffolds were prepared by the crosslinking of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and genipin, and the differentiating BMSCs were characterized against CD44, CD90 and NeuN. The scaffold with 20% PLGA yielded 95% porosity, Young's modulus of 13 MPa, 70% adhesion of BMSCs and 1.6-fold increase in the cell viability over 7-day cultivation. BMSCs without guidance in the PLGA/chitosan scaffolds were prone to differentiate toward osteoblasts with apparent deposition of calcium. When NGF was introduced, an increased weight percentage of PLGA yielded more identified neurons. In addition, mature neurons emerged from the PLGA-rich biomaterials after induction with NGF over 2 days. A proper control over the physical and biomedical property of the scaffolds and the NGF-guided differentiation of BMSCs can be promising for nerve regeneration.

• 出版日期2009-12
• 单位长春大学