Development of Microplate Concentration Measuring Method Aimed to Improve Sensitivity and Simplification

作者：Manaka Atsushi^*; Igarashi Shukuro

来源：Bunseki Kagaku, 2013, 62(9): 811-818.

• visual analysis
• microplate
• small volume sample
• HoLLE

A simplified high-performance determination method using a microplate has been developed as a microanalysis. This review was described with respect to high-sensitive microplate analysis using following reactions, device and method; an autocatalytic reaction with Na2SO3/H2O2, an oxidative reaction from porphyrinogen to porphyrin, microplate immobilized cationic watersoluble porphyrin and homogeneous liquid-liquid extraction. These proposed methods can analyze trace substance (e.g. metal ion, antigen, endocrine disruptor etc.) in small-volume samples. Furthermore, some visual analyses by counting the number of color changes on the microplate were developed. In conclusion, any method is useful and practical for microplate analysis.

• 出版日期2013-9