A novel chemiluminescence quenching method for determination of sulfonamides in pharmaceutical and biological fluid based on luminol-Ag(III) complex reaction in alkaline solution

作者：Sun, Hanwen; Chen, Peiyun^*; Li, Liqing; Cheng, Peng

来源：Drug Testing and Analysis, 2011, 3(5): 300-305.

DOI：10.1002/dta.249

• chemiluminescence
• quenching effect
• Ag(III) complex
• sulfonamides
• luminol: injection
• biological flui

Anovel chemiluminescence (CL) quenching method for the determination of sulfonamides is proposed. The CL reaction between Ag(III) complex [Ag(HIO(6))(2)](5-) and luminol in alkaline solution was investigated. The quenching effect of sulfonamides on CL emission of [Ag(HIO(6))(2)](5-)-luminol system was found. Quenching degree of CL emission was proportional to sulfonamide concentration. The effects of the reaction conditions on CL emission and quenching were examined. Under optimal conditions, the detection limits (s/n = 3) were 7.2, 17 and 8.3 ng/mL for sulfadiazine, sulfameter, and sulfadimethoxine, respectively. The recoveries of the three drugs were in the range of 91.3-110% with RSDs of 1.9-2.7% for urine samples, and 106-112% with RSDs of 1.6-2.8% for serum samples. The proposed method was used for the determination of sulfadiazine at clinically relevant concentrations in real urine and serum samples with satisfactory results.

• 出版日期2011-5
• 单位泰山学院; 河北大学