A simple LC-MS/MS method to determine plasma and cerebrospinal fluid levels of albendazole metabolites (albendazole sulfoxide and albendazole sulfone) in patients with neurocysticercosis

作者：Gonzalez Hernandez Iliana; Isabel Ruiz Olmedo Maria; Cardenas Graciela; Jung Cook Helgi^*

来源：Biomedical Chromatography, 2012, 26(2): 267-272.

DOI：10.1002/bmc.1659

• neurocysticercosis
• albendazole sulfoxide
• albendazole sulfone
• LC-MS/MS
• biological fluids

The development and validation of an LC-MS/MS method for the simultaneous determination of albendazole metabolites (albendazole sulfoxide and albendazole sulfone) in human plasma are described. Samples of 200 mu L were extracted with etherdichloromethanechloroform (60:30:10, v/v/v). The chromatographic separation was performed using a C(18) column with methanolformic acid 20?mmol/L (70:30) as the mobile phase. The method was linear in a range of 20-5000 ng/mL for albendazole sulfoxide and 10-1500 ng/mL for albendazole sulfone. For both analytes the method was precise (RSD %26lt; 12%) and accurate (RE %26lt;7%) with high recovery (%26gt;90%). The method was successfully applied to determine the plasma and cerebrospinal fluid levels of albendazole sulfoxide and albendazole sulfone in patients with subarachnoidal neurocysticercosis who received albendazole at 30 mg/kg per day for 7 days. This LC-MS/MS method yielded a quick, simple and reliable protocol for determining albendazole sulfoxide and albendazole sulfone concentrations in plasma and cerebrospinal fluid samples and is applicable to therapeutic monitoring.

• 出版日期2012-2