Antiproliferative activity of recombinant human interferon-lambda 2 expressed in stably transformed BmN cells

作者：Ye Lu; Jian Zheng Xiao; Yu Xue Ren; Li Cao Guang; De Shen Wei; Liang Gong Cheng^*

来源：African Journal of Biotechnology, 2011, 10(37): 7260-7266.

• Human interferon-lambda 2 protein
• gene expression
• antiproliferative activity

This study aimed at the generation of a stable transformed silkworm BmN cell line which can continuously express human interferon-lambda 2 (IFN-lambda 2) gene, and investigated the antiproliferative activity of this recombinant human IFN-lambda 2. Silkworm BmN cells were transfected with the recombinant vector pIZT/V5-His harboring the human IFN-lambda 2 gene. After the BmN cells were transfected with the pIZT/V5-His- hIFN-lambda 2 vector, the stably transformed BmN cells expressing hIFN-lambda 2 gene were selected using Zeocin. Following two months of screening, the transformed BmN cell line was obtained. Stable transformed BmN cell line can be maintained at a lower Zeocin concentration. The representing 26 kDa protein band of IFN-lambda 2 was detected by SDS-PAGE and Western blotting. The expression level of hIFN-lambda 2, determined by ELISA, was about 8.142 ng in 4 x 10(5) cells. The antiproliferative activity of hIFN-lambda 2 was determined by MTT assay. The 50% inhibitory concentrations (IC(50)) of the recombinant hIFN-lambda 2 on A549 (lung cancer cells), HL60 (acute promyelocytic leukemia cells), BEL-7402 (liver cancer cells) and M231 cells (breast cancer cells) were approximately 3.21, 2.84, 6.29 and 9.32 ng/ml, respectively. In summary, Human IFN-lambda 2 can be stably expressed in the transformed BmN cell line, and the expressed recombinant hIFN-lambda 2 demonstrated antiproliferative activity to tumor cells in vitro.

• 出版日期2011-7-20
• 单位苏州大学