A dominant gdcP-specific marker derived from Moricandia nitens used for introducing the C-3-C-4 character from M-nitens into Brassica crops

作者：Zhang, C; Xu, G; Huang, R; Chen, C; Meng, J^*

来源：Plant Breeding, 2004, 123(5): 438-443.

DOI：10.1111/j.1439-0523.2004.01030.x

• Moricandia nitens
• C-3-C-4 intermediate photosynthesis
• CO2 compensation points
• glycine decarboxylase
• photorespiration
• 5 '-flanking region

Differential localization of decarboxylation and carboxylation in association with Kranz anatomy distinguish species with C-4 or C-3-C-4 intermediate photosynthesis from species with C-3 photosynthesis. Moricandia nitens has a C-3-C-4 intermediate photosynthetic mechanism and yet is closely related to C(3)Brassica species. In order to introduce the C-3-C-4 character from M. nitens into Brassica crops, sesquitetraploids (MACC) were synthesized by crossing Brassica napus (AACC) and a somatic hybrid (M. nitens + B. oleracea, MMCC). Homologous fragments (2966 and 3254 bp) of P-protein gene of glycine decarboxylase (gdcP) were isolated from M. nitens and B. napus, respectively, to develop a PCR-based marker for assisted selection. Reverse transcriptase-polymerase chain reaction (RT-PCR) of the partial encoding regions of both gdcPs indicated that the mRNAs were expressed in the expanded leaf. The characterization of high divergence, but with large conserved island in the regulatory region of the two C-3 species, implies that they may play an important role in maintaining the C-3 photosynthetic pathway. Thereafter, a dominant gene-specific marker was developed within the highly divergent region for subsequent introduction of C-3-C-4 photosynthesis from Moricandia nitens into Brassica crops.

• 出版日期2004-10
• 单位华中农业大学