A microfluidic fluorous solid-phase extraction chip for purification of amino acids

作者：Hu Guoqing; Lee Jacky S H; Li Dongqing^*

来源：Journal of Colloid and Interface Science, 2006, 301(2): 697-702.

DOI：10.1016/j.jcis.2006.05.019

• microfluidics
• fluorous solid-phase extraction
• microbeads
• PDMS

An electrokinetically-driven microfluidic chip was developed to realize beads-based solid-phase extraction (SPE) of amino acids. This chip uses a two-level (deep/shallow) poly(dimethylsiloxane) (PDMS) microchannel network to confine the fluorous reversed-phase silica beads within the SPE chamber. The mixture of fluorous tagged and non-tagged amino acids was carried into the fluorous solid-phase extraction (F-SPE) chamber by electrokinetic pumping and was successfully separated and extracted. By adding a reference material to the sample, the extraction efficiency of the eluted fluorous-tagged amino acid was calculated using the detection results from mass spectrometry (MS). The F-SPE microchips showed good reproducibility and efficiency, yielding an average extraction efficiency of 55% with a RSD of 10.6% under the typical experimental conditions.

• 出版日期2006-9-15