A New Cell Separation Method Based on Antibody-Immobilized Nanoneedle Arrays for the Detection of Intracellular Markers

作者：Kawamura Ryuzo; Miyazaki Minami; Shimizu Keita; Matsumoto Yuta; Silberberg Yaron R; Sathuluri Ramachandra Rao; Iijima Masumi; Ruroda Shun'ichi; Iwata Futoshi; Kobayashi Takeshi; Nakamura Chikashi^*

来源：Nano Letters, 2017, 17(11): 7117-7124.

DOI：10.1021/acs.nanolett.7b03918

• Cell separation
• intracellular marker
• nanoneedle array
• single-cell array
• iPS cells
• high-throughput

Focusing on intracellular targets, we propose a new cell separation technique based on a nano needle array (NNA) device, which allows simultaneous insertion of multiple needles into multiple cells. The device is designed to target and lift ("fish") individual cells from a mixed population of cells on a substrate using an antibody-functionalized NNA. The mechanics underlying this approach were validated by force analysis using an atomic force microscope. Accurate high-throughput separation was achieved using one-to-one contacts between the nano needles and the cells by preparing a single-cell array in which the positions of the cells were aligned with 10,000 nano needles in the NNA. Cell-type-specific separation was realized by controlling the adhesion force so that the cells could be detached in cell-type-independent manner. Separation of nestin-expressing neural stem cells (NSCs) derived from human induced pluripotent stem cells (hiPSCs) was demonstrated using the proposed technology, and successful differentiation to neuronal cells was confirmed.

• 出版日期2017-11