A new case report of urinary tract infection due to KPC-3-producing klebsiella pneumoniae (ST258) in Spain

作者：Sorlozano Puerto Antonio; Esteva Fernandez David; Oteo Iglesias Jesus; Maria Navarro Mari Jose; Gutierrez Fernandez Jose

来源：Archivos Espanoles de Urologia, 2016, 69(7): 437-440.

• Klebsiella pneumoniae
• Carbapenemase
• ST258

OBJECTIVE: We describe the characterization of a new isolated in Spain of Klebsiella pneumoniae ST258 producing KPC-3, carbapenems non-susceptible, recovered from a sample of urine from a patient with urinary tract infection and no history of carbapenems exposure.
METHODS: After the isolation, identification of K. pneumoniae was performed by biochemical tests and mass spectrometry. The carbapenems susceptibility testing was performed by microdilution and E-test in cation-adjusted Mueller-Hinton. The study was completed by Rapidec (R) Carba NP. In order to determine the genetic basis of resistance to carbapenems we used Xpert (R) Carba-R for carbapenemase type and subtype was subsequently analyzed by amplification by PCR and sequencing.
RESULT: We demonstrated by MLST that the strain belonged to the clone of high-risk ST258.
CONCLUSIONS: This is the first characterization, in our media, of a clinical isolated of K. pneumoniae ST258 producing KPC-3 and no history of carbapenems exposure.

• 出版日期2016-9