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摘要
To prevent parthenogenesis effect under embryo formation from sperms and oocyte, this study demonstrates that parthenogenesis of the oocyte of an ICR mouse can be induced with an AC electric field and without sperm insemination. The mouse oocytes were trapped with a positive dielectrophoretic force at the electrodes on an ITO-glass chip. The rates of parthenogenesis were tested under conditions of varied intensity of electric field from 33.35 kV/m to 266.8 kV/m and trapping durations 1, 3, 5 and 10 min. The results indicated that there is no significant statistical difference with varied trapping time (p > 0.1); with AC p-DEP electric activation, there is a significant statistical difference for 133.6 kV/m and 266.8 kV/m of electric field (p = 0.0013 and 0.0001 vs. control group respectively). The rate of parthenogenesis of the control group, oocytes with no electric activation, was 10.17 +/- 0.17% (n = 205). The rates of parthenogenesis were 51.65 +/- 7.8% (n = 27) at electric field 133.6 kV/m and 79.9 +/- 4.85% (n = 35) at 266.8 kV/m. The viability of these oocytes treated with an electric field exceeded 80%; this method of electric activation is hence safe for a mouse oocyte.
- 出版日期2018-6
- 单位清华大学
