Altererythrobacter halimionae sp nov and Altererythrobacter endophyticus sp nov., two endophytes from the salt marsh plant Halimione portulacoides

作者：Fidalgo Catia; Rocha Jaqueline; Martins Ricardo; Proenca Diogo Neves; Morais Paula V; Henriques Isabel^*; Alves Artur

来源：International Journal of Systematic and Evolutionary Microbiology, 2017, 67(8): 3057-3062.

DOI：10.1099/ijsem.0.002079

• Erythrobacteraceae
• endophytic
• halophytes
• taxonomy

Two Gram-negative, rod-shaped, motile bacterial strains, named CPA5(T) and BR75(T), were isolated from the halophyte Halimione portulacoides. Both presented optimum growth at 30 degrees C , pH 7.0-7.5 and 1-2% NaCl (w/v) for strain CPA5(T), and pH 7.5-8.0 and 2% NaCl (w/v) for strain BR75(T). Phylogenetic analyses based on 16S rRNA gene sequences affiliated both strains to the genus Altererythrobacter. CPA5(T) presented highest 16S rRNA gene sequence similarity with Altererythrobacter aestuarii KYW147(T) (96.5 %), followed by Altererythrobacter namhicola KYW48(T) (95.9 %), Novosphingobium indicum H25(T) (95.6 %) and Altererythrobacter oceanensis Y2(T) (95.5 %). BR75(T) displayed highest similarity with Altererythrobacter marensis MSW-14(T) (96.5 %), followed by Altererythrobacter xinjiangensis S3-63(T), Altererythrobacter luteolus SW-109(T) and Altererythrobacter indicus MSSRF26(T) (96.1 %). Neither strain contained Bacteriochlorophyll a. The main fatty acids observed for CPA5(T) were C-17 : 1 omega 6c and summed features 3 (C-16 : 1 omega 7c and/or iso-C-15 (:) (0) 2-OH) and 8 (C-18 : 1 omega 7c and/or C-18 : 1 omega 6c). The latter summed feature was the dominant fatty acid observed for strain BR75(T) as well. The major polar lipids were phosphatidylethanolamine, unidentified phospholipids and unidentified glycolipids for both strains. The predominant ubiquinone was Q-10 for both strains, and the DNA G+C contents were 63.4 mol% and 58.3 mol% for CPA5(T) and BR75(T), respectively. Based on phenotypic and genotypic results, both strains represent novel species belonging to the genus Altererythrobacter for which the names Altererythrobacter halimionae sp. nov. (type strain CPA5(T)=CECT 9130(T)=LMG 29519(T)) and Altererythrobacter endophyticus sp. nov (type strain BR75(T)=CECT 9129(T)=LMG 29518(T)) are proposed.

• 出版日期2017-8