Ultraselective electrochemiluminescence biosensor based on locked nucleic acid modified toehold-mediated strand displacement reaction and junction-probe

作者：Zhang, Xi; Zhang, Jing^*; Wu, Dongzhi; Liu, Zhijing; Cai, Shuxian; Chen, Mei; Zhao, Yanping; Li, Chunyan; Yang, Huanghao; Chen, Jinghua

来源：Analyst, 2014, 139(23): 6109-6112.

DOI：10.1039/c4an01363h

• SINGLE-NUCLEOTIDE POLYMORPHISMS
• ELECTROGENERATED CHEMILUMINESCENCE
• GOLD NANOPARTICLES
• INVASIVE CLEAVAGE
• DNA
• AMPLIFICATION
• FERROCENE
• PLATFORM
• SURFACE

Locked nucleic acid (LNA) is applied in toehold-mediated strand displacement reaction (TMSDR) to develop a junction-probe electro-chemiluminescence (ECL) biosensor for single-nucleotide polymorphism (SNP) detection in the BRCA1 gene related to breast cancer. More than 65-fold signal difference can be observed with perfectly matched target sequence to single-base mismatched sequence under the same conditions, indicating good selectivity of the ECL biosensor.

• 出版日期2014
• 单位福州大学; 福建医科大学; 福建农林大学