科研之友
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摘要
Endothelial HMEC-1 cells incubated with pro-inflammatory cytokine TNF-alpha for 6 and 24 hours were studied as a model of inflammation using Raman imaging. Striking changes in distribution, composition and concentration of cellular lipids were observed after exposure to TNF-a compared to the control. In particular, 3D Raman imaging revealed a significant increase in the amount of lipid entities formed under inflammation. Lipid bodies were randomly distributed in the cytoplasm and two types of droplets were assembled: more saturated one, in spectral characteristics resembling phosphatidylcholine and saturated cholesteryl esters, observed also in the control, and highly unsaturated one, containing also cholesterols, being a hallmark of inflamed cells. The statistical analysis showed that the number of lipid bodies was significantly dependent on the exposure time to TNF-a. Overall, observed formation of unsaturated lipid droplets can be directly correlated with the increase in production of prostacyclins endogenous inflammation mediators.
- 出版日期2017-1-18
