Development and Validation of a Matrix Solid-Phase Dispersion Method to Determine Acrylamide in Coffee and Coffee Substitutes

作者：Dias Soares Cristina M; Alves Rita C; Casal Susana; Oliveira M Beatriz P P; Fernandes Jose Oliveira

来源：Journal of Food Science, 2010, 75(3): T57-T63.

DOI：10.1111/j.1750-3841.2010.01545.x

• acrylamide
• coffee
• coffee substitutes
• GC-MS
• matrix solid-phase dispersion (MSPD)

The present study describes the development and validation of a new method based on a matrix solid-phase dispersion (MSPD) sample preparation procedure followed by GC-MS for determination of acrylamide levels in coffee (ground coffee and brewed coffee) and coffee substitute samples. Samples were dispersed in C(18) sorbent and the mixture was further packed into a preconditioned custom-made ISOLUTE bilayered SPE column (C(18)/Multimode; 1 g + 1 g). Acrylamide was subsequently eluted with water, and then derivatized with bromine and quantified by GC-MS in SIM mode. The MSPD/GC-MS method presented a LOD of 5 mu g/ kg and a LOQ of 10 mu g/kg. Intra and interday precisions ranged from 2% to 4% and 4% to 10%, respectively. To evaluate the performance of the method, 11 samples of ground and brewed coffee and coffee substitutes were simultaneously analyzed by the developed method and also by a previously validated method based in a liquid-extraction (LE) procedure, and the results were compared showing a high correlation between them.

• 出版日期2010-4