Microscale sample preparation for PCR of C. difficile infected stool

作者：Gillers Sara; Atkinson Christopher D; Bartoo Aaron C; Mahalanabis Madhumita; Boylan Michael O; Schwartz John H; Klapperich Catherine^*; Singh Satish K

来源：Journal of Microbiological Methods, 2009, 78(2): 203-207.

DOI：10.1016/j.mimet.2009.05.020

• Sample preparation
• Microfluidics
• Clostridium difficile
• PCR

In this paper, we describe the design of a microfluidic sample preparation chip for human stool samples infected with Clostridium difficile. We established a polymerase chain reaction able to distinguish C difficile in the presence of several other organisms found in the normal intestinal flora. A protocol for on-chip extraction of nucleic acids from clinical samples is described that can detect target DNA down to 5.0 x 10(-3) ng of template. The assay and sample preparation chip were then validated using known positive and known negative clinical samples. The work presented has potential applications in both the developed and developing world.

• 出版日期2009-8