Acid extraction and cloud point preconcentration as sample preparation strategies for cobalt determination in biological materials by thermospray flame furnace atomic absorption spectrometry

作者：Donati GL; Nascentes CC; Nogueira ARA; Arruda MAZ; Nobrega JS^*

来源：Microchemical Journal, 2006, 82(2): 189-195.

DOI：10.1016/j.microc.2006.01.006

• thermospray
• flame atomic absorption spectrometry
• tube in flame
• acid extraction
• cloud point extraction
• cobalt

A thermospray flame furnace atomic absorption spectrometer (TS-FF-AAS) was employed for Co determination in biological materials. Cobalt presents a high atomization temperature and consequently poor sensitivity is obtained without changing its themiochemical behavior. The effect of different complexing agents on sensitivity was evaluated based on the formation of Co volatile compounds. A cloud point procedure was optimized for Co preconcentration for further improvement of sensitivity. Samples were treated with 1 mol l(-1) hydrochloric acid solution for quantitative extraction of Co without simultaneous extraction of Fe, since it is a strong interferent. After the extraction and preconcentration steps, a sample volume of 150 pi was introduced into the hot Ni tube using air as carrier at a flow-rate of 0.4 ml min(-1). The best sensitivity was attained using ammonium pyrrolidinedithiocarbamate (APDC) and Triton X-114 was employed for implementation of the cloud point procedure. The detection limit obtained for Co was 2.1 mu g l(-1) and the standard deviation was 5.8% for a solution containing 100 mu g l(-1) (n = 10). Accuracy was checked using two certified reference materials (tomato leaves and bovine liver) and results were in agreement with certified values at a 95% confidence level. Employing the developed procedure, Co were quantified in different biological materials (plant and animal tissues). The proposed method presents suitable sensitivity for cobalt determination in the quality control of foods.

• 出版日期2006-4