Development of biodegradable PLGA nanoparticles surface engineered with hyaluronic acid for targeted delivery of paclitaxel to triple negative breast cancer cells

作者：Cerqueira Brenda Brenner S; Lasham Annette; Shelling Andrew N; Al Kassas Raida^*

来源：Materials Science & Engineering C-Materials for Biological Applications, 2017, 76: 593-600.

DOI：10.1016/j.msec.2017.03.121

• PLGA nanoparticles
• Hyaluronic acid
• Paclitaxel
• Functionalized nanoparticles
• Degradation studies
• Cytotoxicity

This study aimed at development of poly (lactic-co-glycolic acid) (PLGA) nanoparticles embedded with paclitaxel and coated with hyaluronic acid (HA-PTX-PLGA) to actively target the drug to a triple negative breast cancer cells. Nanoparticles were successfully fabricated using a modified oil-in-water emulsion method. The effect of various formulations parameters on the physicochemical properties of the nanoparticles was investigated. SEM imaging confirmed the spherical shape and nano-scale size of the nanoparticles. A sustained drug release profile was obtained and enhanced PTX cytotoxicity was observed when MDA-MB-231 cells were incubated with the HA-PTX-PLGA formulation compared to cells incubated with the non-HA coated nanoparticles. Moreover, HA-PLGA nanoparticles exhibited improved cellular uptake, based on a possible receptor mediated endocytosis due to interaction of HA with CD44 receptors when compared to non-coated PLGA nanoparticles. The non haemolytic potential of the nanoparticles indicated the suitability of the developed formulation for intravenous administration.

• 出版日期2017-7-1