DPP9 enzymatic activity in hematopoietic cells is dispensable for mouse hematopoiesis

作者：Kim Munkyung; von Muenchow Lilly; Le Meur Thomas; Kueng Benjamin; Gapp Berangere; Weber Delphine; Dietrich William; Kovarik Jiri; Rolink Antonius G; Ksiazek Iwona^*

来源：Immunology Letters, 2018, 198: 60-65.

DOI：10.1016/j.imlet.2018.04.008

• Dipeptidyl peptidase
• DPP9
• DPP9 knock-in enzyme inactive mice
• Hematopoiesis
• Hematopoietic stem cells
• Competitive reconstitution assay

Dipeptidyl peptidase 9 (DPP9) is a ubiquitously expressed intracellular prolyl peptidase implicated in immunoregulation. However, its physiological relevance in the immune system remains largely unknown. We investigated the role of DPP9 enzyme in immune system by characterizing DPP9 knock-in mice expressing a catalytically inactive S729A mutant of DPP9 enzyme (DPP9(ki/ki) mice). DPP9(ki/ki) mice show reduced number of lymphoid and myeloid cells in fetal liver and postnatal blood but their hematopoietic cells are fully functional and able to reconstitute lymphoid and myeloid lineages even in competitive mixed chimeras. These studies demonstrate that inactivation of DPP9 enzymatic activity does not lead to any perturbations in mouse hematopoiesis.

• 出版日期2018-6