bkaR is a TetR-type repressor that controls an operon associated with branched-chain keto-acid metabolism in Mycobacteria

作者：Balhana Ricardo J C; Swanston Sade N; Coade Stephen; Withers Mike; Sikder Mahmudul Hasan; Stoker Neil G; Kendall Sharon L^*

来源：FEMS Microbiology Letters, 2013, 345(2): 132-140.

DOI：10.1111/1574-6968.12196

• Rv2506
• MSMEG_4718
• accA1
• accD1
• transcriptional regulation
• microarray

This study describes how bkaR, a highly conserved mycobacterial TetR-like transcriptional repressor, regulates a number of nearby genes that have associations with branched-chain keto-acid metabolism. bkaR (MSMEG_4718) was deleted from the nonpathogenic species Mycobacterium smegmatis, and changes in global gene expression were assessed using microarray analysis and reporter gene studies. bkaR was found to directly control the expression of 10 genes in M.smegmatis, and its ortholog in Mycobacterium tuberculosis (Rv2506) is predicted to control at least 12 genes. A conserved operator motif was identified, and binding of purified recombinant M.tuberculosis BkaR to the motif was demonstrated. Analysis of the stoichiometry of binding showed that BkaR binds to the motif as a dimer.

• 出版日期2013-8
• 单位河北医科大学