A TiO2-Sol-Gel derived Prussian blue nanoparticles-based glucose biosensor

作者：Li Jianping; Wang Sufen; Ayoub Iyad M; Yang Catherine F

来源：2nd IEEE Sensors Applications Symposium, 2007-02-06 to 2007-02-08.

• glucose biosensor
• titanic dioxide Sol-Gel
• Prussian blue nanoparticles

A highly sensitive and selective glucose biosensor was designed by immobilizing glucose oxidase (GOD) on a Prussian blue nanoparticles (nanoPBs) modified glassy carbon electrode using a novel titanic dioxide Solution-Gelation (TiO2-Sol-Gel) technique. Glucose measurement was based on the level of hydrogen peroxide (H2O2) produced concomitantly with the reaction of glucose and oxygen mediated by GOD. NanoPBs catalyze this reaction efficiently by reducing the reaction potential and improving GOD activity. The biosensor showed voltaic response to H2O2 in a linear range of 5.0 x 10(-7) to 4.0 x 10(-4) mol/L and amperometric response to glucose in a linear range of 1.0 x 10(-4) to 2.4 X 10(-2) mmol/L with fine reproducibility (relative standard deviation varied from 3.6% to 4.2%). This nanoPBs-based biosensor also exhibits high recovery (97% similar to 101.5%) for measuring blood glucose levels and the developed method is applicable for large-scaled manufacturing of disposable biosensors.

• 出版日期2007
• 单位桂林理工大学