Modeling of lutein production by heterotrophic Chlorella in batch and fed-batch cultures

作者：Wu, Zheng Yun; Shi, Chun Lei; Shi, Xian Ming^*

来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2007, 23(9): 1233-1238.

DOI：10.1007/s11274-007-9354-2

• lutein production
• xanthophyll
• Chlorella pyrenoidosa
• heterotrophic culture
• kinetic model

Chlorella is a promising alternative resource of lutein (xanthophyll) production as it can be cultivated heterotrophically in fermentors. In this paper, a kinetic model for lutein production by heterotrophic Chlorella pyrenoidosa was developed based on batch cultivations in 250-ml flasks and a 19-1 fermentor. The model was validated by experimental data from two fed-batch cultivations performed in the same fermentor. The dynamic behavior of lutein production by C pyrenoidosa with various concentrations of glucose and nitrogen was analyzed based on the kinetic model. Model-based analyses suggested that glucose concentrations between 5 and 24 g/l and nitrogen concentrations between 0.7 and 12 g/l during the cultivation were favorable for lutein production by heterotrophic C pyrenoidosa. It also showed that fed-batch cultivations are more suitable for efficient production of lutein than batch ones. The results obtained in this study may contribute to commercial lutein production by heterotrophic Chlorella.

• 出版日期2007-9
• 单位上海交通大学