Molecular Cloning and Functional Characterization of a Novel Gene Encoding Human Prostaglandin Carrier, hPrC

作者：Kobayashi Yasuna; Nojima Junko; Ohbayashi Masayuki; Kohyama Noriko; Yamamoto Toshinori^*

来源：Journal of the Pharmaceutical Society of Japan, 2011, 131(10): 1493-1501.

DOI：10.1248/yakushi.131.1493

• drug transporter
• prostaglandin
• organic solute

In the present study, we isolated and determined the pharmacological characteristics of a novel gene encoding the human prostaglandin carrier (hPrC). The isolated cDNA consisted of 1431 base pairs that encoded a 477-amino acid protein, and we found that isolated hPrC does not belong to any drug transporter families. RT-PCR analysis revealed that the hPrC mRNA is expressed in various human tissues ubiquitously. When expressed in Xenopus laevis oocytes, hPrC mediated the transport of [H-3] prostaglandin E-2 (PGE(2)) in a sodium-independent manner. The uptake of [3H] PGE2 was not trans-stimulated by PG analogous. Although there are several PG transporters such as multidrug resistance-associated protein 4 (MRP4), organic cation transporter 1 (OCT1) [solute carrier (SLC) 22AI], organic anion transporter 1-3 (OAT1-3) [SLC22A6-8], OAT4 [SLCH], OATP-1 (LST-1) [SLCOIBI], OATP2B1 [SLCO2B1], OATP2A1 (PGT) [SLCO2AI], OATP4A1 (OATP-E) [SLCO4A1] have been isolated and well characterized, our findings suggest that hPrC functions as a novel transport peptide responsible for PG uptake. Our results should provide insight into the novel mechanism of the PG transport in the human body.

• 出版日期2011-10