Antioxidant Activity and Mechanism of Tetrahydroamentoflavone in vitro

作者：Li, Xican^*; Han, Weijuan; Mai, Wenqiong; Wang, Li

来源：Natural Product Communications, 2013, 8(6): 787-789.

DOI：10.1177/1934578x1300800626

• Tetrahydroamentoflavone
• Antioxidant
• Radical-scavenging
• Chelating
• Biflavonoid

(2S,2 '' S)-Tetrahydroamentoflavone (THA) is a typical biflavonoid. In this study, we systematically evaluated its antioxidant activity by various antioxidant assays in vitro. The IC50 values of THA were 4.8 /- 0.3, 743.2 /- 49.5, 35.5 /- 1.9, 165.7 /- 22.8, 4.4 /- 0.2, and 77.1 /- 2.2 mu g/mL, respectively, for superoxide (center dot O-2(-)) radical-scavenging, Fe2 -chelating, Cu2 -chelating, DPPH center dot (1,1-diphenyl-2-picrylhydrazyl radical) scavenging, ABTS center dot(double dagger) (3-ethylbenzthiazoline-6-sulfonic acid radical) scavenging, and Ce2 -reducing power. The average ratio value of IC50,(Trolox):IC50,(THA) was calculated as 2.54, suggesting that THA possesses 2.54 times the total antioxidant level than the standard antioxidant Trolox. THA exerts its antioxidant activity in vitro through metal-chelating, and radical-scavenging, which is via donating a hydrogen atom (H.) and an electron (e). Its antioxidant activity can be responsible for its pharmacological effect and make it an attractive natural antioxidant.

• 出版日期2013-6
• 单位广州中医药大学