Development and design of five multi-locus microsatellite PCR panels for population genetic surveys of white hake (Urophycis tenuis) in the Northwest Atlantic

作者：Roy D^*; Paterson I G; Hurlbut T R; Ruzzante D E

来源：Conservation Genetics Resources, 2010, 2(1): 45-49.

DOI：10.1007/s12686-009-9140-6

• White hake
• Urophycis tenuis
• Microsatellite
• Multiplex
• Northwest Atlantic
• Gulf of St. Lawrence

We describe five multi-locus PCR panels incorporating twelve new and seven previously characterized microsatellites, useful for population genetic surveys of white hake. All but one locus are polymorphic with an average allelic richness of 14.26 (1.00-50.45 range) and mean observed and expected heterozygosities of 0.680 (0.221-0.888 range) and 0.738 (0.269-0.953 range), respectively. Three loci do not fit Hardy-Weinberg expectations suggesting internal genetic structuring within the sampled population, consistent with previous reports. These multi-locus panels will be used to determine population structure of white hake in the Northwest Atlantic, clarify important factors regulating its population dynamics and help establish much needed conservation efforts to recover this species in this region.

• 出版日期2010-12