Antioxidant effect of ethanolic extract of Piper betle Linn. (Paan) on erythrocytes from patients with HbE-beta thalassemia

作者：Srimani Phalguni; Mandal Goutam; Ganguly Sudipto; Saha Piu; Sen Rupashree; De Rajib; Chatterjee Arnab; Bhattacharyya Maitrayee; Chaudhuri Utpal; Bandyopadhyay Susanta K; Chatterjee Mitali^*

来源：Indian Journal of Biochemistry & Biophysics, 2009, 46(3): 241-246.

• HbE beta thalassemia
• Reactive oxygen species (ROS)
• Piper betle Linn.
• Anti-oxidant
• Erythrocytes

HbE-beta thalassemia is caused by an interaction between HbE and defective P globin gene of thalassemia. Repeated blood transfusions cause an iron overload, triggering an enhanced generation of free radicals. In the present study, the anti-oxidant property of ethanolic extract of the leaves of Piper betle Linn. (PB) was evaluated in the erythrocytes from patients with HbE-beta thalassemia. In patients with HbE-beta thalassemia (n = 30) and age- and sex-matched healthy individuals (n = 30), the baseline level of reactive oxygen species (ROS) and free radical scavenging activity in the erythrocytes was measured by flow cytometry using dihydrodichlorofluorescein diacetate (H(2)DCFDA), in terms of the geometric mean fluorescence channel (GMFC). The baseline generation of ROS was significantly higher in the erythrocytes from patients with HbE-beta thalassemia, as compared to healthy volunteers, the GMFC being 67.20 +/- 4.64 vs. 23.03 +/- 1.88 (p<0.001), which was effectively decreased by PB. Similarly, H(2)O(2) (0.5-1.0 mM) induced a higher increase in the GMFC in the erythrocytes from patients with HbE-beta thalassemia, as compared to controls which was effectively reduced by PB. Taken together, PB showed promising anti-oxidant activity against the erythrocytes from patients with HbE-beta thalassemia.

• 出版日期2009-6
• 单位河北医科大学