Development of bio-based fine chemical production through synthetic bioengineering

作者：Hara Kiyotaka Y; Araki Michihiro; Okai Naoko; Wakai Satoshi; Hasunuma Tomohisa; Kondo Akihiko^*

来源：Microbial Cell Factories, 2014, 13(1): 173.

DOI：10.1186/s12934-014-0173-5

• Fine chemical
• Synthetic bioengineering
• Metabolic engineering
• Enzymatic synthesis
• Microbial fermentation
• Bioinformatics

Fine chemicals that are physiologically active, such as pharmaceuticals, cosmetics, nutritional supplements, flavoring agents as well as additives for foods, feed, and fertilizer are produced by enzymatically or through microbial fermentation. The identification of enzymes that catalyze the target reaction makes possible the enzymatic synthesis of the desired fine chemical. The genes encoding these enzymes are then introduced into suitable microbial hosts that are cultured with inexpensive, naturally abundant carbon sources, and other nutrients. Metabolic engineering create efficient microbial cell factories for producing chemicals at higher yields. Molecular genetic techniques are then used to optimize metabolic pathways of genetically and metabolically well-characterized hosts. Synthetic bioengineering represents a novel approach to employ a combination of computer simulation and metabolic analysis to design artificial metabolic pathways suitable for mass production of target chemicals in host strains. In the present review, we summarize recent studies on bio-based fine chemical production and assess the potential of synthetic bioengineering for further improving their productivity.

• 出版日期2014-12-14