Dipeptidyl peptidase-4 expression in pancreatic tissue from patients with congenital hyperinsulinism

作者：Rahman Sofia A; Senniappan Senthil; Sherif Maha; Tahir Sophia; Hussain Khalid^*

来源：International Journal of Clinical and Experimental Pathology, 2015, 8(7): 8199-8208.

• DPP-4
• CHI
• hypoglycaemia

Congenital hyperinsulinism (CHI) is caused by unregulated insulin release and leads to hyperinsulinaemic-hypoglycaemia (HH). Glucagon like peptide-1 (GLP-1), glucose-dependent insulinotropic peptide (GIP), peptide YY (PYY) and the enzyme; dipeptidyl peptidase-4 (DPP-4) all regulate appetite and glucose homeostasis. These proteins have been identified as possible contributors to HH but the mechanism remains poorly understood. We aimed to look at the expression pattern of pancreatic DPP-4 in children with focal and diffuse CHI (FCHI and DCHI, respectively). Using immunohistochemistry; we determined DPP-4 expression patterns in the pancreas of CHI patients. DPP-4 was found to be expressed in the pancreatic beta, alpha and delta-cells in and around the focal area. However, it was predominantly co-localised with beta-cells in the paediatric tissue samples. Additionally, proliferating beta-cells expressed DPP-4 in DCHI, which was absent in the FCHI pancreas. Insulin was found to be present in the exocrine acini and duct cells of the DCHI pancreas suggestive of exocrine to endocrine transdifferentiation. Furthermore, 6 medically-unresponsive DCHI pancreatic samples showed an up-regulation of total pancreatic DPP-4 expression. In conclusion; the expression studies have shown DPP-4 to be altered in HH, however, further work is required to understand the underlying role for this enzyme.

• 出版日期2015