Na,K-ATPase Isozymes in Colorectal Cancer and Liver Metastases

作者：Baker Bechmann Marc; Rotoli Deborah; Morales Manuel; del Carmen Maeso Maria; del Pino Garcia Maria; Avila Julio; Mobasheri Ali; Martin Vasallo Pablo^*

来源：Frontiers in Physiology, 2016, 7: 9.

DOI：10.3389/fphys.2016.00009

• Na/K-ATPase isozymes
• sodium pump isozymes
• colorectal cancer
• colorectal cancer liver metastases
• Na/K-ATPase isoforms colorectal cancer immunohistochemistry

The goal of this study was to define Na,K-ATPase alpha and beta subunit isoform expression and isozyme composition in colorectal cancer cells and liver metastases. The alpha 1, alpha 3, and beta 1 isoforms were the most highly expressed in tumor cells and metastases; in the plasma membrane of non-neoplastic cells and mainly in a cytoplasmic location in tumor cells. alpha 1 beta 1 and alpha 1 beta 1 isozymes found in tumor and metastatic cells exhibit the highest and lowest Na+ affinity respectively and the highest K+ affinity. Mesenchymal cell isozymes possess an intermediate Na+ affinity and a low K+ affinity. In cancer, these ions are likely to favor optimal conditions for the function of nuclear enzymes involved in mitosis, especially a high intra-nuclear K+ concentration. A major and striking finding of this study was that in liver, metastasized CRC cells express the eiNil isozyme. Thus, the alpha 3 beta 1 isozyme could potentially serve as a novel exploratory biomarker of CRC metastatic cells in liver.

• 出版日期2016-1-29