Analyzing Diurnal and Age-Related Pheromone Emission of the Olive Fruit Fly, Bactrocera oleae by Sequential SPME-GCMS Analysis

作者：Levi Zada Anat^*; Nestel David; Fefer Daniela; Nemni Lavy Esther; Deloya Kahane Inbal; David Maayan

来源：Journal of Chemical Ecology, 2012, 38(8): 1036-1041.

DOI：10.1007/s10886-012-0167-x

• Olive fruit fly
• Bactrocera oleae
• Sequential SPME-GCMS analysis
• Autosampler
• Pheromone chirality
• Diptera
• Tephritidae

The olive fruit fly, Bactrocera oleae (Diptera: Tephritidae), uses 1,7-dioxaspiro[5.5]undecane ("olean"), produced primarily by females, as a sex pheromone. We used sequential solid phase microextraction-gas chromatography mass spectrometry (SPME-GCMS) analysis to show that female olive flies release about 1000 ng of pheromone at the onset of scotophase for several weeks, while males release about 1/100 as much during the first week after eclosion. The present research demonstrates details of employing SPME-GCMS with the partially known pheromone system of the olive fruit fly as a model for pheromone identification and diurnal release patterns in insects, especially fruit flies. The sequential SPME-GCMS method will readily allow detection and semi-quantification of semiochemicals released by insects in minute amounts throughout the diurnal cycle.

• 出版日期2012-8