NRP1 knockdown promotes the migration and invasion of human neuroblastoma-derived SK-N-AS cells via the activation of 1 integrin expression

作者：Ishizuka Yoshiaki; Koshinaga Tsugumichi; Hirano Takayuki; Nagasaki Maeoka Eri; Watanabe Yosuke; Hoshi Reina; Yoshizawa Shinsuke; Sugito Kiminobu; Kawashima Hiroyuki; Uekusa Shota; Fukuda Noboru; Soma Masayoshi; Fujiwara Kyoko^*

来源：International Journal of Oncology, 2018, 53(1): 159-166.

DOI：10.3892/ijo.2018.4397

• neuroblastoma
• neuropilin 1
• 1 integrin
• cell migration
• cell invasion

Neuropilin 1 (NRP1) is a transmembrane glycoprotein, which regulates many aspects of cellular function by functioning as co-receptor of various ligands. Recent studies have suggested that NRP1 promotes tumorigenesis, not only by activating the growth of tumor vessels, but also by activating the growth or migration of tumor cells themselves. The present study was performed to elucidate the roles of NRP1 in the development and/or progression of neuroblastoma (NB). In contrast to previous observations in various types of cancer, the analysis of public datasets indicated that lower levels of NRP1 expression were significantly associated with a shorter survival period of patients with NB. Consistent with this finding, wound-healing assay and Matrigel invasion assay revealed that NB cells in which NRP1 was knocked down exhibited increased migratory and invasive abilities. Further analyses indicated that 1 integrin expression was markedly increased in NB cells in which NRP1 was knocked down, and NB cells in which 1 integrin was knocked down exhibited decreased migratory and invasive abilities. The results presented herein indicate that NRP1 exerts tumor suppressive effects in NB, at least in part by regulating the expression of 1 integrin.

• 出版日期2018-7