Development of a high performance electrochemical cofactor regeneration module and its application to the continuous reduction of FAD

作者：Ruinatscha Reto; Buehler Katja^*; Schmid Andreas

来源：Journal of Molecular Catalysis B: Enzymatic , 2014, 103: 100-105.

DOI：10.1016/j.molcatb.2013.07.003

• Cofactor regeneration
• Electrochemistry
• Mass transfer
• Oxidoreductases
• Three-dimensional electrodes

Technical reactor limitations and low productivities have been shown to limit the implementation of electroenzymatic syntheses beyond lab-scale. One possible solution is a continuous flow-through reactor based on electrochemical plate and 6 frame filter press cells, as proposed in this study. With the aim of maximizing electroenzymatic productivities, the developed reactor set-up allows high electrochemical cofactor regeneration rates using porous, three-dimensional reticulated vitreous carbon electrodes with exceptionally large surface areas up to 19,685 m(2) m(-3). This system provides increased mass transfer rates and flavin adeninedinucleotide (FAD) was reduced at rates up to 93 mM h(-1). The electrochemical FAD reduction was coupled to the styrene monooxygenase (StyA) catalyzed (S)-epoxidation of styrene. Electroenzymatic productivities increased with FAD reduction rates up to 1.3 mM h(-1). This set-up now set the stage for efficient in vitro FAD regeneration and allows a broad electrochemical application of flavin dependent enzymes as biocatalysts.

• 出版日期2014-5
• 单位TU Dortmund