A gold nanoparticle-modified indium tin oxide microelectrode for in-channel amperometric detection in dual-channel microchip electrophoresis

作者：Zhu, Gangzhi; Song, Qianhui; Liu, Wenfang; Yan, Xingxing; Xiao, Jian^*; Chen, Chuanpin^*

来源：Analytical Methods, 2017, 9(29): 4319-4326.

DOI：10.1039/c7ay01008g

• CONTACTLESS CONDUCTIVITY DETECTION
• FILM COATED GLASS
• CAPILLARY-ELECTROPHORESIS
• ELECTROCHEMICAL DETECTION
• DIRECT ELECTRODEPOSITION
• CHIRAL SELECTORS
• CARBON
• SENSOR
• SENSITIVITY
• PERFORMANCE

A modified indium tin oxide (ITO) microelectrode detector integrated with a dual-channel polydimethylsiloxane (PDMS) microchip is proposed for in-channel amperometric detection in microchip electrophoresis (ME). The gold nanoparticle (GNP)-modified ITO-coated poly-ethylene terephthalate film electrode was fabricated via a photolithography process and direct electrochemical deposition. This electrode fabrication method reduced the cost of this microchip a lot. The electroplating conditions for the GNP-modified ITO electrode were optimized through evaluating the effect on the detection sensitivity and separation efficiency of a dual-channel ME system. Under the optimal conditions, the performance of the new ME amperometric detection system has been demonstrated by analyzing a mixture of p-aminophenol, o-aminophenol and m-aminophenol, which achieved the detection limits of 0.24 mu M, 0.41 mu M and 0.42 mu M, respectively. Finally, the successful detection of antibiotics demonstrated the potential of this dual-channel microchip system for clinical application.

• 出版日期2017-8-7
• 单位中南大学; 海口市人民医院