Antibacterial, modulatory activity of antibiotics and toxicity from Rhinella jimi (Stevaux, 2002) (Anura: Bufonidae) glandular secretions

作者：Sales Debora Lima^*; Bezerra Morais Braga Maria Flaviana; Lucas dos Santos Antonia Thassya; Targino Machado Antonio Judson; de Araujo Filho Joao Antonio; Dias Diogenes de Queiroz; Bezerra da Cunha Francisco Assis; Saraiva Rogerio de Aquino; Alencar de Menezes Irwin Rose; Melo Coutinho Henrique Douglas; Martins Costa Jose Galberto; Ferreira Felipe Silva; da Nobrega Alvese Romulo Romeu; Almeida Waltecio de Oliveira

来源：Biomedicine & Pharmacotherapy, 2017, 92: 554-561.

DOI：10.1016/j.biopha.2017.05.098

• Poisons
• Parotoid gland
• Antimicrobial activity
• Bacterial resistance
• Modulation of antibiotics
• Toxicity
• Cytotoxicity

The increase in microorganisms with resistance to medications has caused a strong preoccupation within the medical and scientific community. Animal toxins studies, such as parotoid glandular secretions from amphibians, possesses a great potential in the development of drugs, such as antimicrobials, as these possess bioactive compounds. It was evaluated Rhinella jimi (Stevaux, 2002) glandular secretions against standard and multi-resistant bacterial strains; the effect of secretions combined with drugs; and determined the toxicity using two biologic in vivo models, and a in vitro model with mice livers. Standard strains were used for the determination of the Minimum Inhibitory Concentration (MIC), while for the modulatory activity of antibiotics, the clinical isolates Escherichia coli 06, Pseudomonas aeruginosa 03 and Staphylococcus aureus 10 were used. Modulatory activity was evaluated by the broth microdilution method with aminoglycosides and beta-lactams as target antibiotics. The secretions in association with the antibiotics have a significant reduction in MIC, both the aminoglycosides and b-lactams. The toxicity and cytotoxicity results were lower than the values used in the modulation. R. jimi glandular secretions demonstrated clinically relevant results regarding the modulation of the tested antimicrobials.

• 出版日期2017-8