Nanofiber-based sutures induce endogenous antimicrobial peptide

作者：Chen, Shixuan; Ge, Liangpeng; Gombart, Adrian F.; Shuler, Franklin D.; Carlson, Mark A.; Reilly, Debra A.; Xie, Jingwei^*

来源：Nanomedicine, 2017, 12(21): 2597-2609.

DOI：10.2217/nnm-2017-0161

• 25-hydroxyvitamin D-3
• co-delivery
• electrospun nanofibers
• pam3Cys-Ser-(Lys)4 peptide
• surgical site infection
• sutures

Aim: The aim of this study was to develop nanofiber-based sutures capable of inducing endogenous antimicrobial peptide production. Methods: We used co-axial electrospinning deposition and rolling to fabricate sutures containing pam3CSK4 peptide and 25-hydroxyvitamin D-3 (25D(3)). Results: The diameters and mechanical properties of the sutures were adjustable to meet the criteria of United States Pharmacopeia designation. 25D(3) exhibited a sustained release from nanofiber sutures over 4 weeks. Pam3CSK4 peptide also showed an initial burst followed by a sustained release over 4 weeks. The co-delivery of 25D(3) and pam3CSK4 peptide enhanced cathelicidin antimicrobial peptide production from U937 cells and keratinocytes compared with 25D(3) delivery alone. In addition, the 25D(3)/pam3CSK4 peptide co-loaded nanofiber sutures did not significantly influence proliferation of keratinocytes, fibroblasts, or the monocytic cell lines U937 and HL-60. Conclusion: The use of 25D(3)/pam3CSK4 peptide co-loaded nanofiber sutures could potentially induce endogenous antimicrobial peptide production and reduce surgical site infections.

• 出版日期2017-11
• 单位重庆市畜牧科学院