A novel immunochromatographic system for easy-to-use detection of group 1 avian influenza viruses with acquired human-type receptor binding specificity

作者：Watanabe Yohei a^*; Ito Tetsuo; Ibrahim Madiha S; Arai Yasuha; Hotta Kozue; Hoang Vu Mai Phuong; Nguyen Le Khanh Hang; Le Quynh Mai; Soda Kosuke; Yamaoka Masaoki; Poetranto Emmanuel Djoko; Wulandari Laksmi; Hiramatsu Hiroaki; Daidoji Tomo; Kubota Koketsu Ritsuko; Sriwilaijaroen Nongluk; Nakaya Takaaki; Okuno Yoshinobu; Takahashi Tadanobu; Suzuki Takashi; Ito Toshihiro; Hotta Hak; Yamashiro Tetsu; Hayashi Tsukasa; Morita Kouichi; Ikuta Kazuyoshi; Suzuki Yasuo

来源：Biosensors and Bioelectronics, 2015, 65: 211-219.

DOI：10.1016/j.bios.2014.10.036

• Immunochromatographic strip test
• H5N1 avian influenza virus
• Group 1 influenza A virus
• Detection of receptor binding specificity
• Sialylglycopolymer
• Pandemic potential

A switch of viral hemagglutinin receptor binding specificity from bird-type alpha 2,3- to human-type alpha 2,6-linked sialic acid is necessary for an avian influenza virus to become a pandemic virus. In this study, an easy-to-use strip test to detect receptor binding specificity of influenza virus was developed. A biotinylated anti-hemagglutinin antibody that bound a broad range of group 1 influenza A viruses and latex-conjugated alpha 2,3 (blue) and alpha 2,6 (red) sialylglycopolymers were used in an immunochromatographic strip test, with avidin and lectin immobilized on a nitrocellulose membrane at test and control lines, respectively. Accumulation of a sialylglycopolymer-virus-antibody complex at the test line was visualized by eye. The strip test could be completed in 30 min and did not require special equipment or skills, thereby avoiding some disadvantages of current methods for analyzing receptor binding specificity of influenza virus. The strip test could detect the receptor binding specificity of a wide range of influenza viruses, as well as small increases in the binding affinity of variant H5N1 viruses to alpha 2,6 sialylglycans at viral titers > 128 hemagglutination units. The strip test results were in agreement with those of ELISA virus binding assays, with correlations > 0.95. In conclusion, the immunochromatographic strip test developed in this study should be useful for monitoring potential changes in the receptor binding specificity of group 1 influenza A viruses in the field.

• 出版日期2015-3-15