An IL-6/IL-6 soluble receptor (IL-6R) hybrid protein (H-IL-6) induces epo-independent erythroid differentiation in human CD34(+) cells

作者：Baiocchi M^*; Marcucci I; Rose John S; Serlupi Crescenzi O; Biffoni M

来源：Cytokine, 2000, 12(9): 1395-1399.

DOI：10.1006/cyto.2000.0723

• CD34+
• cord blood
• erythropoiesis
• interleukin 6
• stem cell factor

H-IL-6 is a hybrid protein constructed to contain IL-6 and its soluble receptor linked by a flexible peptide chain. Here we show that H-IL-6 strongly enhances proliferation of human CD34(+) cells in serum-free liquid culture, and that the majority of the cells generated belong to the erythroid lineage, being positive for the marker Glycophorin A. Conversely, H-IL-6 does not increase the number of myeloid, CD13-positive cells. Comparable effects are observed on progenitors from cord blood and adult peripheral blood. Therefore, H-IL-6 triggers an erythroid-inducing signal in haematopoietic progenitor cells, independently from erythropoietin (EPO).

• 出版日期2000-9