High-speed label-free functional photoacoustic microscopy of mouse brain in action

作者：Yao, Junjie; Wang, Lidai; Yang, Joon-Mo; Maslov, Konstantin I.; Wong, Terence T. W.; Li, Lei; Huang, Chih-Hsien; Zou, Jun; Wang, Lihong V.^*

来源：Nature Methods, 2015, 12(5): 407-+.

DOI：10.1038/nmeth.3336

• IN-VIVO
• HEMODYNAMIC-CHANGES
• OXYGEN
• TISSUE
• CORTEX

We present fast functional photoacoustic microscopy (PAMAM) for three-dimensional high-resolution, high-speed imaging of the mouse brain, complementary to other imaging modalities. We implemented a single-wavelength pulse-width-based method with a one-dimensional imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution. We applied PAMAM to image the vascular morphology, blood oxygenation, blood flow and oxygen metabolism in both resting and stimulated states in the mouse brain.

• 出版日期2015-5