科研之友
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摘要
Background: Given that Delta(9)-tetrahydrocannabinol, the active constituent of cannabis, has been shown to greatly reduce driving ability, thus being linked to many drug driving accidents, its reliable detection is of great importance. Results: An optimised carbon paste electrode, fabricated from graphite powder and mineral oil, is utilised for the sensitive detection of Delta(9)-tetrahydrocannabinol (THC) in both aqueous solutions of pH 10.0 and in synthetic saliva solutions. "Absorptive Stripping Voltammetry" is exploited to that effect and the paste is used to pre-concentrate the carbon paste electrode with the target molecule. Practical limits of detection of 0.50 mu M and 0.10 mu M are determined for THC in stationary and stirred aqueous borate buffer solutions, respectively. Theoretical limits of detection are also calculated; values of 0.48 nM and 0.41 nM are determined for stationary and stirred THC aqueous borate buffer solutions, respectively. THC concentrations as low as 0.50 mu M are detected in synthetic saliva olutions. The sensitivity of the sensor was 0.12 mu A mu M-1, 0.84 mu A mu M-1 and 0.067 mu A mu M-1 for the stationary buffer, the stirred buffer and the saliva matrix, respectively. Conclusions: "Absorptive Stripping Voltammetry" can be reliably applied to the detection of Delta(9)-tetrahydrocannabinol, after suitable optimisation of the assay. Usefully low practical limits of detection can be achieved.
- 出版日期2015-7-1
