Leukemia inhibitory factor-expressing human embryonic lung fibroblasts as feeder cells for human embryonic germ cells

作者：Li Fangfei; Liu Yonggang; Chen Dilong; Lin Xuemei; Li Jing; Wang Jianwei; Peng Yan; Wang Shali; Wang Yaping^*

来源：Cells Tissues Organs, 2007, 186(4): 221-228.

DOI：10.1159/000107637

• feeder layer
• human embryonic germ cells
• human embryonic stem cells
• leukemia inhibitory factor
• primordial germ cells

A robust culture system is critical for maintaining both proliferation and the developmental potential of human embryonic germ ( hEG) cells. Here, we use human embryonic lung fibroblasts ( hELF) overexpressing leukemia inhibitory factor (LIF) as feeder cells to support the self-renewal of hEG cells. We examine the morphology, gene expression, and developmental potential of hEG cells grown on a feeder layer of LIF-expressing hELF (hELF/lif) cells. hEG cells were positive for alkaline phosphatase (AP), stage-specific embryonic antigen (SSEA)-1, SSEA- 4, tumor rejection antigen (TRA)-1-60, and TRA- 1 - 81. In addition, hEG cells maintained on hELF/lif expressed higher levels of pluripotency genes such as Oct4 and Nanog. In addition, hEG cells maintained on hELF/lif cells gave rise to differentiated tissues when grown as embryoid bodies, consistent with the broad developmental potential of the starting population. Our results suggest that a hELF/lif feeder layer can support the proliferation of hEG cells, and that LIF signaling plays an essential role in this process. This human-derived culture system provides an attractive alternative to more commonly used mouse-derived feeder layers for use in clinical applications.

• 出版日期2007
• 单位重庆医科大学