Active Vitamin D prevents anti-osteogenic effect of AGEs on human osteoblasts

作者：Oelzner P^*; Franke S; Hofmann G; Wolf G

来源：Osteologie, 2013, 22(2): 129-135.

• AGEs
• vitamin D
• osteoblasts
• mRNA expression

Objective: To investigate whether 1,25-dihydroxyvitamin D-3 (1,25D(3)) is able to prevent advanced glycation end products (AGE)-induced alterations of osteoblasts (OB).
Methods: Human OB were isolated and cultured from bone tissue of ten patients with knee osteoarthritis and joint replacement. Cells from passages three to seven were treated with control bovine serum albumin (Co-BSA), AGE-BSA or AGE-BSA supplemented with 1,25D(3) in two different concentrations (medium concentrations: 5 mg/ml AGE-BSA and Co-BSA, respectively; 100 pmol/l and 500 pmol/l 1,25D(3)). mRNA and protein expression of bone alkaline phosphatase (bALP), collagen type 1 (Col1) and osteocalcin (OC) were investigated by real-time PCR and Western Blot-analysis, respectively.
Results: AGE-BSA reduced mRNA expression of bALP, Col1 and OC in comparison to Co-BSA significantly. Addition of 1,25D(3) in both concentrations completely prevented the AGE-BSA-induced suppression and resulted in a further increase of mRNA and protein expression of bALP, Col1 and OC.
Discussion: 1,25D(3) in a physiological concentration prevents anti-osteogenic effects of AGEs on human OB. Therefore, 1,25D(3) treatment should be beneficial in diseases associated with AGE accumulation, impaired bone formation and vitamin D deficiency such as senile osteoporosis and rheumatoid arthritis.

• 出版日期2013