Emergence of carbapenem-resistant Enterobacter cloacae carrying IMP-26 metallo-β-lactamase in class 1 integron and TEM-104 extended-spectrum β-Lactamase in conjugative plasmid

作者：Ye, Qing; Shao, Wenxia; Wang, Qingqing^*; Chen, Xuejun; Li, Jianping; Zhou, Mingming

来源：African Journal of Microbiology Research, 2012, 6(13): 3186-3191.

DOI：10.5897/AJMR11.1477

• Metallo-beta-lactamases
• carbapenemases
• Enterobacter cloacae

A clinical isolate of Enterobacter cloacae exhibiting reduced susceptibility to carbapenem and a positive EDTA-disc synergy test was studied for carbapenemase production. MICs were determined with standard procedures. Isoelectric focusing of cell extracts was used for detection of beta-lactamases. PCR assays with primers specific for the bla(IMP-26) gene and the conserved segments of class 1 integrons and sequence analyses were carried out to identify the gene and to map the metallo-beta-lactamase encoding integron. Transferability of the gene was assessed with conjugation experiments. To identify the location of the bla(TEM-104) gene, conjugation experiments and plasmid electrophoresis were carried out and amplified by PCR. A carbapenem-resistant E. cloacae was found to carry IMP-26 metallo-beta-lactamase in class 1 integron and TEM-104 extended-spectrum beta- lactamase in conjugative plasmid. Existence of multiple resistant genes on plasmids and integrons may pose a serious epidemiological, clinical, and public health threat.

• 出版日期2012-4-9
• 单位浙江大学; 杭州市第一人民医院