Anti-allergenic activity of polymethoxyflavones from Kaempferia parviflora

作者：Kobayashi Shoko^*; Kato Taro; Azuma Toshiaki; Kikuzaki Hiroe; Abe Keiko

来源：Journal of Functional Foods, 2015, 13: 100-107.

DOI：10.1016/j.jff.2014.12.029

• Antiallergic activity
• Degranulation
• Kaempferia parviflora
• Polymethoxyflavone
• RBL-2H3
• Zingiberaceae

The inhibitory effects of 13 polymethoxyflavones (PMFs) obtained from Kaempferia paruiflora extracts were evaluated on RBL-2H3 cell degranulation. These flavones suppressed antigeninduced degranulation in RBL-2H3 cells. 5-Hydroxy-3,7,4',-trimethoxyflavone (KP02) and 5,3'-dihydroxy-3,7,4',-trimethoxyflavone (KP10) showed potent inhibitory activities. KP10 significantly suppressed calcium ionophores, such as A23187 and 2,5-ditert-butylhydroquinone, which promoted calcium influx from the outside of cells and from the endoplasmic reticulum, respectively. However, these activities did not fully explain the PMFs' effects. Immunoblot analysis of KPO2 and KP10 showed that the inhibitory effects were attributable to the downregulation of PLC gamma 1 kinase phosphorylation in the signaling pathways involved in degranulation. The phosphorylation levels of Syk were also suppressed by the addition of KP10. KP02 and KP10 treatment to the cells did not affect the high affinity IgE receptor (Fc epsilon RI) levels inside RBL-2H3 cells, but they suppressed the Fc epsilon RI levels in the plasma membrane. Therefore, K. parviflora PMFs have multiple degranulation mechanisms to suppress RBL-2H3 cells and would be beneficial for alleviating type I allergy symptoms.

• 出版日期2015-3