Dietary luteolin protects against HgCl2-induced renal injury via activation of Nrf2-mediated signaling in rat

作者：Tan, Xiao; Liu, Biying; Lu, Jingjing; Li, Siyu; Baiyun, Ruiqi; Lv, Yueying; Lu, Qian; Zhang, Zhigang^*

来源：Journal of Inorganic Biochemistry, 2018, 179: 24-31.

DOI：10.1016/j.jinorgbio.2017.11.010

• Luteolin
• HgCl2
• Renal injury
• Nrf2
• NF-kappa B
• Apoptosis

Luteolin (Lut) belongs to the flavonoid family with various beneficial bioactivities. Here, we investigated whether Lut attenuate mercuric chloride (HgCl2)-induced renal injury in rat. We found that oral gavage administration of Lut (80 mg/kg) alleviated anemia and renal histology upon HgCl2 treatment (80 mg/L). Lut also significantly reduced HgCl2-induced oxidative stress and inflammatory, presenting as the reduced malondialdehyde (MDA) formation, increased glutathione (GSH) level, and inhibited activation of nuclear factor kappa B (NF-kappa B). Moreover, Lut protected renal cells from HgCl2-induced apoptosis, as assessed by Terminal deoxynucleotidyl transferase dUNT nick end labeling (TUNEL) assay and the protein levels of B-cell lymphoma gene 2 (Bcl-2), B-cell lymphoma-extra large (Bcl-xL), Bcl-2-associated X protein (Bax), and p53. Interestingly, Lut reduced renal mercuric accumulation in rat. Furthermore, Lut increased nuclear translocation of the nuclear factor-erythroid-2-related factor 2 (Nrf2), and subsequent protein expression of the antioxidant enzymes, heme oxygenase-1 (HO-1) and nicotinamide adenine dinucleotide phosphatase: quinone-acceptor 1 (NQO1). Our results suggest that Lut suppress HgCl2-induced renal injury via activation of Nrf2 signaling pathway.

• 出版日期2018-2
• 单位东北农业大学