Determination of synephrine in weight-loss products using high performance liquid chromatography with acidic potassium permanganate chemiluminescence detection

作者：Slezak Teo; Francis Paul S^*; Anastos Nicole; Barnett Neil W

来源：Analytica Chimica Acta, 2007, 593(1): 98-102.

DOI：10.1016/j.aca.2007.04.038

• synephrine
• biogenic amines
• adrenergic amines
• herbal extracts
• dietary supplements
• monolithic column chromatography
• acidic potassium permanganate chemiluminescence

Adrenergic amines found in extracts of Citrus aurantium (bitter orange) evoke analytically useful chemiluminescence with acidic potassium permanganate in the presence of polyphosphates. From corrected chemiluminescence spectra, the wavelength of maximum intensity for these reactions was 680 +/- 5 nm and, using flow injection analysis methodology, limits of detection for synephrine, octoparnine, tyramine and hordenine were found to be between 1 x 10(-9) and 1 x 10(-8) M. We have applied this method of detection to the rapid determination of synephrine in dietary supplements using monolithic column chromatography.

• 出版日期2007-6-12
• 单位迪肯大学