Molecular identification of 'Candidatus Phytoplasma fraxini' in murta and peony in Chile

作者：Arismendi Nolberto; Gonzalez Flor; Zamorano Alan; Andrade Nancy; Pino Ana Maria; Fiore Nicola^*

来源：Bulletin of Insectology, 2011, 64: S95-S96.

• Ugni molinae
• peony
• nested-PCR
• phytoplasmas
• in silico RFLP
• sequencing

Plants of murta (Ugni molinae Turcz.) and peony (Paeonia lactiflora Pall.), exhibiting disease symptoms suggestive of possible phytoplasma infection were observed in Chile. Leaves were collected from six plants per species (three with and three without symptoms) and main leaf midribs were used for phytoplasma testing. Nested polymerase chain reaction (PCR) amplification allowed the detection of phytoplasmas in samples from plants that showed symptoms, but not in those from the asymptomatic ones. Cloning, sequencing and in silico restriction fragment length polymorphism (RFLP) of the 16S RRNA gene allowed identification of the phytoplasmas into ribosomal subgroup 16SrVII-A for both species.

• 出版日期2011