Autofluorescence spectroscopy for evaluating dysplasia in colorectal tissues

作者：Luo, Xiang-jian; Zhang, Bo; Li, Jian-guo; Luo, Xiang-an; Yang, Li-fang

来源：Zeitschrift für Medizinische Physik, 2012, 22(1): 40-47.

DOI：10.1016/j.zemedi.2011.10.010

• Autofluorescence spectroscopy
• dysplasia
• colorectal tissue

The aim of this study was to assess the applicability of autofluorescence (AF) spectroscopy as a method for the diagonosis of normal, benign and malignant of dysplasia in colorectal tissues experimentally By improvement of optical design in laser pulse generator, wavelength-adjustable output was acquired and the optimal wavelength was defined as 380 nm. With 380-nm pulsed laser excitation, AF spectra of normal, benign and malignant colorectal tissues were recorded in the spectra region from 460-570 Inn in vitro. The spectral analysis for discrimination among the different types of tissues was carried out using principal component analysis (PCA)-based neural networks algorithm. The performance of analysis was pretty good with sensitivity, specificity and accuracy found to be 100%, 90% and 96.7%, respectively. The AF spectroscopy may serve as an excellent tool for the evaluation of dysplasia in colorectal clinical diagnosis.

• 出版日期2012
• 单位中南大学