Determination of Starch Lysophospholipids in Rice Using Liquid Chromatography-Mass Spectrometry (LC-MS)

作者：Liu, Lei; Tong, Chuan; Bao, Jinsong^*; Waters, Daniel L. E.; Rose, Terry J.; King, Graham J.

来源：Journal of Agricultural and Food Chemistry, 2014, 62(28): 6600-6607.

DOI：10.1021/jf500585j

• rice
• lysophospholipid
• LC-MS
• starch lipid
• amylose-lipid complex
• grain quality

Acquiring a complete understanding of rice starch lysophospholipids (LPLs), their biosynthetic pathways and genetic diversity, and the influence of genotype by environment interactions has been hampered by the lack of efficient high-throughput extraction and analysis methods. It was hypothesized a single-step aqueous n-propanol extraction combined with liquid chromatography mass spectrometry (LC-MS) could be employed to analyze starch LPLs in white rice. The investigation found different grinding methods showed little effect on the final LPL detected, and a simple single-step extraction with 75% n-propanol (8 mL/0.15 g) heated at 100 degrees C for 2 h was as effective as an onerous multistep extraction method. A LC-MS method was optimized to simultaneously quantify 10 major LPLs in rice starch within 15 mm. This method enables total and individual starch LPL analysis of a large number of rice samples at little cost. This approach could be applied to starch LPLs in other cereals.

• 出版日期2014-7-16
• 单位浙江大学