科研之友
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摘要
Simple screening of acid-triggered reactions of methoxybenzyl alcohols led to the development of a novel colorirnetric hydrophobic benzyl alcohol (HBA) tag. HBA tag-3 (14) retained high solubility in less polar solvents and excellent precipitation properties in polar solvents. Our routine procedure for tag-assisted liquid phase peptide synthesis was applied using HBA tag-3 (14), and an effective synthesis of beta-sheet breaker peptide iA beta 5 (4) was achieved. The tagged peptides showed a vivid blue color under acidic conditions both on TLC plates and in solution, enabling quantitative assay.
- 出版日期2015-9-4
