科研之友
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摘要
Astrocytes possess many of the same signalling molecules as neurons. However, the role of astrocytes in information processing, if any, is unknown. Using electrophysiological and imaging methods, we report the first evidence that astrocytes modulate neuronal sensory inhibition in the rodent thalamus. We found that mGlu2 receptor activity reduces inhibitory transmission from the thalamic reticular nucleus to the somatosensory ventrobasal thalamus (VB): mIPSC frequencies in VB slices were reduced by the Group II mGlu receptor agonist LY354740, an effect potentiated by mGlu2 positive allosteric modulator (PAM) LY487379 co-application (30 nM LY354740: 10.0 1.6% reduction; 30 nM LY354740 & 30 pM LY487379: 34.6 5.2% reduction). We then showed activation of mGlu2 receptors on astrocytes: astrocytic intracellular calcium levels were elevated by the Group II agonist, which were further potentiated upon mG1u2 PAM co-application (300 nM LY354740: ratio amplitude 0.016 0.002; 300 nM LY354740 & 30 pM LY487379: ratio amplitude 0.035 0.003). We then demonstrated mGlu2-dependent astrocytic disinhibition of VB neurons in vivo: VB neuronal responses to vibrissae stimulation trains were disinhibited by the Group II agonist and the mGlu2 PAM (LY354740: 156 12% of control; LY487379: 144 10% of control). Presence of the glial inhibitor fluorocitrate abolished the mG1u2 PAM effect (91 5% of control), suggesting the mGlu2 component to the Group II effect can be attributed to activation of mG1u2 receptors localised on astrocytic processes within the VB. Gating of thalamocortical function via astrocyte activation represents a novel sensory processing mechanism. As this thalamocortical circuitry is important in discriminative processes, this demonstrates the importance.
- 出版日期2017-7-15
